Characterization of the Pinus massoniana Transcriptional Response to Bursaphelenchus xylophilus Infection Using Suppression Subtractive Hybridization

نویسندگان

  • Liang Xu
  • Zhen-Yu Liu
  • Kai Zhang
  • Quan Lu
  • Jun Liang
  • Xing-Yao Zhang
چکیده

Pine wilt disease (PWD) caused by pine wood nematode (PWN), Bursaphelenchus xylophilus, is the most destructive diseases of pine and poses a threat of serious economic losses worldwide. Although several of the mechanisms involved in disease progression have been discovered, the molecular response of Pinus massoniana to PWN infection has not been explored. We constructed four subtractive suppression hybridization cDNA libraries by taking time-course samples from PWN-inoculated Masson pine trees. One-hundred forty-four significantly differentially expressed sequence tags (ESTs) were identified, and 124 high-quality sequences with transcriptional features were selected for gene ontology (GO) and individual gene analyses. There were marked differences in the types of transcripts, as well as in the timing and levels of transcript expression in the pine trees following PWN inoculation. Genes involved in signal transduction, transcription and translation and secondary metabolism were highly expressed after 24 h and 72 h, while stress response genes were highly expressed only after 72 h. Certain transcripts responding to PWN infection were discriminative; pathogenesis and cell wall-related genes were more abundant, while detoxification or redox process-related genes were less abundant. This study provides new insights into the molecular mechanisms that control the biochemical and physiological responses of pine trees to PWN infection, particularly during the initial stage of infection.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Identification and Characterization of a Bursaphelenchus xylophilus (Aphelenchida: Aphelenchoididae) Thermotolerance-Related Gene: Bx-HSP90

Temperatures directly influence the distribution and intensity of pine wilt disease caused by the pine wood nematode, Bursaphelenchus xylophilus. To date, however, little is known about the causation and mechanism of this influence. The molecular chaperone HSP90 is a key component that contributes to survival in the abiotic stress response. In this study, we investigated the relationship betwee...

متن کامل

Identification of genes upregulated by pinewood nematode inoculation in Japanese red pine.

Pine wilt disease caused by the pinewood nematode (PWN), Bursaphelenchus xylophilus (Steiner et Buhrer) Nickle, has destroyed huge areas of pine forest in East Asia, including Japan, China and Korea. No protection against PWN has been developed, and the responses of pine trees at the molecular level are unrecorded. We isolated and analyzed upregulated or newly induced genes from PWN-inoculated ...

متن کامل

Effect of Bursaphelenchus xylophilus infection on leaf photosynthetic characteristics and resource‐use efficiency of Pinus massoniana

Pine wilt disease (PWD) is considered as the most destructive forest-invasive alien species in China. We measured gas exchange parameters and foliar carbon isotope ratios (δ13C) of different infection phases of Masson pine in order to investigate the effect of Bursaphelenchus xylophilus infection on photosynthetic responses and resource-use efficiency. The results showed that net photosynthetic...

متن کامل

Characterization of a Nonparasitic Isolate of Bursaphelenchus xylophilus.

Bursaphelenchus xylophilus isolate MPSy-1av was subcultured from pathotype MPSy-1. MPSy-1av is nonparasitic and does not establish in Pinus sylvestris, P. strobus, P. nigra, or P. taeda. This isolate produces ethanol as an end product of carbohydrate metabolism, whereas its parent pathotype, MPSy-1, does not. Alcohol dehydrogenase activity was easily detectable in homogenates of MPSy-1av but ba...

متن کامل

Quantitative Classification and Environmental Interpretation of Secondary Forests 18 Years After the Invasion of Pine Forests by Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae) in China

With growing concerns over the serious ecological problems in pine forests (Pinus massoniana, P. thunbergii) caused by the invasion of Bursaphelenchus xylophilus (the pine wood nematode), a particular challenge is to determine the succession and restoration of damaged pine forests in Asia. We used two-way indicator species analysis and canonical correlation analysis for the hierarchical classif...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره 14  شماره 

صفحات  -

تاریخ انتشار 2013